| Annotated protein: | Alpha-actinin-1 (Alpha-actinin cytoskeletal isoform) (F-actin cross-linking protein) (Non-muscle alpha-actinin-1). Gene symbol: ACTN1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9Z1P2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ACTN1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic actin cytoskeleton (GO:0098871) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Nuriya M, et al. "Phosphorylation-dependent interactions of alpha-Actinin-1/IQGAP1 with the AMPA receptor subunit GluR4" J Neurochem. 2005 Oct;95(2):544-52 PMID:16190873 |
| Figure(s): | Figures 1-4 |
| Annotation description: | Figure 1,2: yeast two-hybrid and co-immunoprecipitation in HEK293T cells showed α-Actinin-1 (ACTN1) interacts with GluR4 (GRIA4). Figure 3: "Myc-tagged full-length α-Actinin-1 and IQGAP1 clones were transfected into low density hippocampal primary neuronal cultures together with GFP-tagged GluR4, and the cellular localization of these proteins was analyzed by immunocytochemistry 2 days after transfection. GFP-GluR4, myc-α-Actinin-1 and myc-IQGAP1 all showed punctate signals throughout the dendrite, both in the shafts and the putative spines (Fig. 3). Moreover, clusters of GFP-GluR4 overlapped extensively with those of myc-α-Actinin-1 (Fig. 3a) and myc-IQGAP1 (Fig. 3b). Co-localizations patterns were even clearer with the surface population of GFP-GluR4 that was specifically labeled by live staining." Figure 4f: GRIA4 and ACTN1 co-localized in hippocampal neuronal cultures that were double-labeled with anti-GluR4 and anti-α-Actinin. |
| Evidence tracking, Biological System: | Non-neuronal tissue Cell-free system Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Y2H IP + WB/MSMS |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5049 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |