| Annotated protein: | Chronophin (EC 3.1.3.16) (EC 3.1.3.74) (Pyridoxal 5'-phosphate phosphatase) (Pyridoxal phosphate phosphatase) (PLP phosphatase). Gene symbol: PDXP. Taxonomy: Mus musculus (Mouse). Uniprot ID: P60487 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PDXP |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Kim JE, et al. "PLPP/CIN regulates bidirectional synaptic plasticity via GluN2A interaction with postsynaptic proteins" Sci Rep. 2016 May 23;6:26576 PMID:27212638 |
| Figure(s): | Figure 1, S3 |
| Annotation description: | Figure 1, S3: Comparing the morphology of hippocampal dendritic spines in PLPP/CIN (aka. Chronophin, gene PDXP) knockouts and transgenic mice overexpressing human PLPP/CIN to wildtype mice, there was no effect on dendritic spine density, an increase in spine head width/size in knockouts and a decrease in the transgenic mice. Thus, PLPP/CIN is a negative regulator of dendritic spine (head) size. Figure 2, 3: PLPP/CIN disruption affects both LTP and LTD. Figure 5: PLPP/CIN expression modulates the binding of regulatory molecules (GluN1, PSD95, CaMKII, pCaMKII, calcineurin) to GluN2A. Taken together, authors infer that PLPP/CIN (aka. Chronophin, gene PDXP) is localized to the postsynapse and regulates structural plasticity (Fig. 8). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Microscopy (generic) IP + WB/MSMS |
| Non-tracable Author Statement (NAS): | This annotation required some inference over additional information/literature, and is not solely based on observations made in the annotated paper by its original authors. |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | of dendritic spine morphology in PLPP/CINTg and PLPP/CIN-/- mice. As compared to WT animals, the spines in PLPP/CINTg mice show thin necks with very small heads, while PLPP/CIN-/- mice show gigantic spines @ PMID:28993724 PLPP/CIN interacts with NEDD4-2 @ PMID:31320629 |
| SynGO annotation ID: | 5047 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |