| Annotated protein: | Rho GTPase activating protein 12. Gene symbol: ARHGAP12. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: D3ZDI5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ARHGAP12 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse assembly (GO:0150052) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Ba W, et al. "ARHGAP12 Functions as a Developmental Brake on Excitatory Synapse Function" Cell Rep. 2016 Feb 16;14(6):1355-1368 PMID:26854232 |
| Figure(s): | Figure 2, 6 |
| Annotation description: | Figure 2: ARHGAP12 negatively regulates spine morphology. "Compared to neurons expressing GFP alone, Arhgap12-overexpressing CA1 pyramidal neurons displayed a significant decrease in both spine density and volume ... In addition, we found that elevated ARHGAP12 levels significantly increased the percentage of immature spines in CA1 neurons. These observations are consistent with experiments in which reduced Rac1 activity has been coupled to reduced spine density and size (Haditsch et al., 2009)." Figure 6: ARHGAP12 regulates synaptic structure and function via distinct domains. "compared the effects of regulating dendritic spine morphology between ARHGAP12 WT and two mutants, Arhgap12_R596Q and Arhgap12_Δ1-156aa. Spine structure analysis revealed that expression of the Arhgap12_R596Q mutant in CA1 pyramidal neurons failed to mimic the phenotype observed by expressing Arhgap12 WT, namely, decreased spine density and volume compared to GFP control ... These data indicate that the GAP activity of ARHGAP12, but not its interaction with CIP4, is required to regulate dendritic spine morphology." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Two-photon microscopy |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5046 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |