| Annotated protein: | Neurogenic locus notch homolog protein 1 (Notch 1) (Motch A) (mT14) (p300) [Cleaved into: Notch 1 extracellular truncation (NEXT); Notch 1 intracellular domain (NICD)]. Gene symbol: NOTCH1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q01705 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NOTCH1 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Brai E, et al. "Notch1 Regulates Hippocampal Plasticity Through Interaction with the Reelin Pathway, Glutamatergic Transmission and CREB Signaling" Front Cell Neurosci. 2015 Nov 26;9:447 PMID:26635527 |
| Figure(s): | Figure 1 |
| Annotation description: | Figure 1a: immunogold EM of hippocampal cryosections using a specific antibody against the cytoplasmic tail of Notch1 showed localization to the postsynaptic density Figure 1c,d: Fluorescent immunolabeling on primary neuronal cultures shows that Notch1 and ApoER2 are partially co-expressed in NMDAR1 positive puncta. Similarly, dendritic (spine) puncta for Notch1 and Dab1 are observed. Further functional assays confirm that Notch1 not only co-localized with these proteins but also interferes with the Reelin pathway and regulates NMDA transmission, adding further credibility to postsynaptic localization. Based on the known transmembrane topology of this protein, "integral component of ... membrane" term was selected. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5040 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |