| Annotated protein: | Low-density lipoprotein receptor-related protein 8 (LRP-8) (Apolipoprotein E receptor 2). Gene symbol: LRP8. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q924X6 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ LRP8 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic neurotransmitter receptor activity (GO:0098962) |
| Synapse type(s): | Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Beffert U, et al. "Modulation of synaptic plasticity and memory by Reelin involves differential splicing of the lipoprotein receptor Apoer2" Neuron. 2005 Aug 18;47(4):567-79 PMID:16102539 |
| Figure(s): | Figure 3, 6 |
| Annotation description: | Figure 3a-c: field recordings from Schaffer collateral synapses in wildtype and exon-19 mutant mice showed no significant differences of synaptic transmission, paired-pulse facilitation, and TBS-induced LTP between wild-type and Apoer2 (ex19) and Apoer2 (Δex19) mice. Figure 3d-e: In response to Reelin, Apoer2 (ex19) mice showed a robust increase in LTP induction (Figure 3D), which was completely abrogated in the neuroanatomically indistinguishable Apoer2 (Δex19) mutant (Figure 3E). Figure 3f: Reelin potentiates NMDA receptor-mediated pEPSPs recorded in area CA1 when AMPA receptors are blocked with CNQX. Wild-type and Apoer2 (ex19) mice showed a similarly significant increase in NMDA receptor potentials upon Reelin perfusion, which was maintained longer in the Apoer2 (ex19) mutants. By contrast, the Apoer2 (Δex19) mutant showed no elevation of NMDA receptor-dependent potentials above baseline synaptic responses. Figure 6: immunoprecipitation assays in HEK293 cells showed Apoer2 (LRP8) can interact with proteins PSD-95 (DLG4) and NR2A. Authors conclude: "Taken together, these data indicate that LTP enhancement by Reelin involves a molecular mechanism that is dependent upon the alternatively spliced exon 19 (of Apoer2). In addition, this mechanism involves, at least in part, the modulation of NMDA receptor functions." |
| Evidence tracking, Biological System: | Intact tissue Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Over-expression |
| Evidence tracking, Experiment Assay: | Field recordings IP + WB/MSMS |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Hippocampal LTP induction is defective in ApoER2 knockout mice @ PMID:12167620 |
| SynGO annotation ID: | 5039 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |