| Annotated protein: | Low-density lipoprotein receptor-related protein 8 (LRP-8) (Apolipoprotein E receptor 2). Gene symbol: LRP8. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q924X6 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ LRP8 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Beffert U, et al. "Modulation of synaptic plasticity and memory by Reelin involves differential splicing of the lipoprotein receptor Apoer2" Neuron. 2005 Aug 18;47(4):567-79 PMID:16102539 |
| Figure(s): | Figure 6, 7 |
| Annotation description: | Figure 6: immunoprecipitation assays in HEK293 cells showed Apoer2 (LRP8) can interact with proteins PSD-95 (DLG4) and NR2A. Figure 7a: Apoer2 is enriched in PSD biochemical fractions similar to PSD-95 from rat brains. Figure 7b-g: Apoer2 and PSD-95 co-localize in cultured (rat) hippocampal neurons. Figure 7h,i: Immunogold EM showed Apoer2 is localized to the PSD in CA1 hippocampi Based on the known transmembrane topology of this protein, "integral component of ... membrane" term was selected. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Antibody (detection) Over-expression |
| Evidence tracking, Experiment Assay: | Electron Microscopy Western blot Biochemical fractionation (generic) IP + WB/MSMS Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5038 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |