| Annotated protein: | SH3 domain-containing kinase-binding protein 1 (Regulator of ubiquitous kinase) (Ruk) (SH3-containing, expressed in tumorigenic astrocytes). Gene symbol: SH3KBP1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q925Q9 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SH3KBP1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | hippocampus, GABAergic striatum, glutamatergic |
| Annotated paper: | Shimokawa N, et al. "CIN85 regulates dopamine receptor endocytosis and governs behaviour in mice" EMBO J. 2010 Jul 21;29(14):2421-32 PMID:20551902 |
| Figure(s): | Figure 1, 4, 5 |
| Annotation description: | Figure 1b: "In primary hippocampal neurons derived from wild-type rat, CIN85 (SH3KBP) is localized to dendritic spines, in which it is accumulated in post-synaptic compartments, co-localizing with F-actin and PSD-95 (DLG4), closely juxtaposing the pre-synaptic synaptophysin protein" Figure 1c: "CIN85 (SH3KBP) localizes to actin-positive, spine-like structures in dendrites of primary rat striatal neurons. Cells were fixed and stained with antibodies against CIN85 (SH3KBP) and with rhodamine phalloidin" Figure 1d,e: "biochemical fractions showed CIN85 (SH3KBP) was enriched in PSD fractions together with marker protein PSD95 (DLG4)." Figure 4: "CIN85 co-clusters with D2 dopamine receptors (D2DRs) at punctuate synapse-like structures in primary rat striatal neurons" Figure 5: "CIN85 co-immunoprecipitates with D2DRs, the endocytic proteins endophilin and dynamin, as well as the post-synaptic density protein PSD-95, in wild type, but not in CIN85Δex2 knockout samples" |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal IP + WB/MSMS |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5034 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |