| Annotated protein: | SH3 domain-containing kinase-binding protein 1 (Regulator of ubiquitous kinase) (Ruk) (SH3-containing, expressed in tumorigenic astrocytes). Gene symbol: SH3KBP1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q925Q9 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SH3KBP1 |
| Ontology domain: | Cellular Component |
| SynGO term: | synapse (GO:0045202) |
| Annotated paper: | Kawata A, et al. "CIN85 is localized at synapses and forms a complex with S-SCAM via dendrin" J Biochem. 2006 May;139(5):931-9 PMID:16751601 |
| Figure(s): | Figure 4 |
| Annotation description: | Figure 4a: Both CIN85 (SH3KBP1) and dendrin (DDN) were detected in various synaptic biochemical fractions of rat brain, including the postsynaptic density fraction. Figure 4b: CIN85 (SH3KBP1) synaptic localization was shown using immunofluorescence labeling with confocal imaging of CIN85 and synaptophysin (SYP) in rat hippocampal neurons. The imaging resolution is too low to narrow down localization to pre- and/or post-synapse. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | CIN85 (SH3KBP1) and D2 dopamine receptors (D2DR) co-localize at punctuate synapse-like structures in primary rat striatal neurons @ PMID:20551902 |
| SynGO annotation ID: | 5033 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |