| Annotated protein: | Serine/threonine-protein kinase PAK 3 (EC 2.7.11.1) (Beta-PAK) (CDC42/RAC effector kinase PAK-B) (p21-activated kinase 3) (PAK-3). Gene symbol: PAK3. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q61036 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PAK3 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | forebrain, glutamatergic |
| Annotated paper: | Hayashi ML, et al. "Altered cortical synaptic morphology and impaired memory consolidation in forebrain- specific dominant-negative PAK transgenic mice" Neuron. 2004 Jun 10;42(5):773-87 PMID:15182717 |
| Figure(s): | Fig. 1b |
| Annotation description: | Phosphorylated forms of PAK1, PAK2, and PAK3 (p-PAK1/2/3) were all present in the PSD fraction, while un-phosphorylated PAK1/3 (Up-PAK1/3) were nearly absent from the PSD fraction. As detected by the p-PAK antibody, the amount of p-PAK1 was much higher than that of p-PAK2 and p-PAK3. 29/5/2017 Pim - PAK3 is not a presynaptic protein that precipitates with the PSD through tight transsynaptic linkage. This is evidenced by the localization of pan-PAK in spines (this paper) and the PAK3 localization in spines (PMID:22815483) 29/8/2017 Pim - Authors state: "p-PAK predominantly localizes in the cell soma, dendrites, and dendritic spines while it is largely excluded from the axons and boutons of mature cortical neurons" based on IF data. However, this is with a pan-PAK antibody and cannot be used w/o the biochemical purification data. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Wide-field fluorescence Western blot Biochemical fractionation (generic) |
| Annotator(s): | Alexandros Kanellopoulos (ORCID:0000-0002-2094-7491) Vittoria Mariano (ORCID:0000-0002-9848-0262) Achsel Tilmann (ORCID:0000-0002-1190-4481) Claudia Bagni (ORCID:0000-0002-4419-210X) |
| Lab: | Department of Fundamental Neurosciences, University of Lausanne, CH-1006 Lausanne, Switzerland; Dept Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy |
| Additional literature: | PAK3 and PAK1 are coexpressed in single neurons, colocalize in dendritic spines, co-purified with the postsynaptic density, and coimmunoprecipitate in brain extracts. Fig. 5 @ PMID:22815483 |
| SynGO annotation ID: | 502 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |