| Annotated protein: | G protein-activated inward rectifier potassium channel 2 (GIRK-2) (Inward rectifier K(+) channel Kir3.2) (Potassium channel, inwardly rectifying subfamily J member 6). Gene symbol: KCNJ6. Taxonomy: Mus musculus (Mouse). Uniprot ID: P48542 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ KCNJ6 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Fajardo-Serrano A, et al. "Association of Rgs7/Gbeta5 complexes with Girk channels and GABAB receptors in hippocampal CA1 pyramidal neurons" Hippocampus. 2013 Dec;23(12):1231-45 PMID:23804514 |
| Figure(s): | Figure 7 |
| Annotation description: | Figure 7: EM of immunoperoxidase labeled Girk2 (KCNJ6) showed both pre- and post-synaptic localization. "Electron micrographs of the distal part of the stratum radiatum of the hippocampal CA1 region showing colocalization for Rgs7 or Gβ5 (GNB5) with Girk2 (KCNJ6), as detected using a pre-embedding immunogold method. Labelling is shown with immunogold for the Rgs7 or Gβ5, and with immunoperoxidase for the Girk2 subunit. Postsynaptically, Rgs7 (panel A) or Gβ5 (panel B) were seen in dendritic spines (s) of presumed pyramidal cells immunopositive for Girk2. Presynaptically, Rgs7 (panel C) or Gβ5 (panel D) were detected in Girk2-positive axon terminal (at) establishing asymmetrical contact with a dendritic spine (s)." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | characterization of the polyclonal Girk2 antibody @ PMID:18088366 characterization of the polyclonal Girk2 antibody @ PMID:19558451 |
| SynGO annotation ID: | 5019 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |