| Annotated protein: | Serine/threonine-protein kinase PAK 1 (EC 2.7.11.1) (Alpha-PAK) (CDC42/RAC effector kinase PAK-A) (p21-activated kinase 1) (PAK-1) (p65-PAK). Gene symbol: PAK1. Taxonomy: Mus musculus (Mouse). Uniprot ID: O88643 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PAK1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | forebrain, glutamatergic |
| Annotated paper: | Hayashi ML, et al. "Altered cortical synaptic morphology and impaired memory consolidation in forebrain- specific dominant-negative PAK transgenic mice" Neuron. 2004 Jun 10;42(5):773-87 PMID:15182717 |
| Figure(s): | Fig. 1 |
| Annotation description: | Biochemical fractionation of mouse forebrain followed by Western blot analysis reveals the presence of phosphorylated PAK1 in the PSD fraction. 29/8/2017 Pim - Authors state: "p-PAK predominantly localizes in the cell soma, dendrites, and dendritic spines while it is largely excluded from the axons and boutons of mature cortical neurons" based on IF data. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Wide-field fluorescence Western blot Biochemical fractionation (generic) |
| Annotator(s): | Alexandros Kanellopoulos (ORCID:0000-0002-2094-7491) Vittoria Mariano (ORCID:0000-0002-9848-0262) Achsel Tilmann (ORCID:0000-0002-1190-4481) Claudia Bagni (ORCID:0000-0002-4419-210X) |
| Lab: | Department of Fundamental Neurosciences, University of Lausanne, CH-1006 Lausanne, Switzerland; Dept Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy |
| Additional literature: | PAK1 is expressed in single neurons (immunohistochemistry), localize in dendritic spines, purified with the postsynaptic density (Western blot), in mouse brain extracts. Fig. 5. @ PMID:22815483 |
| SynGO annotation ID: | 500 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |