Annotated protein:Cerebellin-1 (Brain protein D3) (Precerebellin) [Cleaved into: Cerebellin (CER); [des-Ser1]-cerebellin (des-Ser1-cerebellin)]. Gene symbol: CBLN1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9R171
antibody wiki:
SynGO gene info:SynGO data @ CBLN1
Ontology domain:Biological Process
SynGO term:trans-synaptic signaling, modulating synaptic transmission (GO:0099550)
Synapse type(s):cerebellum, glutamatergic
Annotated paper:Dai J, et al. "Distinct neurexin-cerebellin complexes control AMPA- and NMDA-receptor responses in a circuit-dependent manner" Elife. 2022 Oct 7;11:e78649 PMID:36205393
Figure(s):Figure 1-8
Annotation description:Nrxn1/3 - Cbln1/2 trans-synaptic signaling complexes differentially control NMDA- and AMPA-receptors in different synapses in diverse neural circuits without regulating synapse or spine formation. The Neurexin alternative splicing site 4 (SS4) is critical to these signaling pathways.

Summary of paper figures;

Figure 1: Constitutive Cbln2 deletion increases AMPAR-EPSCs and suppresses NMDAR-EPSCs at CA1→subiculum synapses formed on both burst- and regular-spiking subiculum neurons, and additionally blocks NMDAR-dependent LTP in regular-spiking neurons without affecting cAMP-dependent LTP in burst-spiking neurons.

Figure 2: Constitutive Cbln2 deletion impairs contextual memory in the two-chamber avoidance test.

Figure 3: Constitutive Cbln2 deletion does not alter the overall synapse density in the subiculum.

Figure 4: Constitutive Cbln2 deletion occludes regulation of postsynaptic AMPAR- and NMDAR-EPSCs by presynaptic Nrxn1SS4+ and Nrxn3SS4+, respectively.

Figure 5: Cbln1 and Cbln2 double KO in the subiculum phenocopies the Cbln2 single KO at CA1→subiculum synapses.

Figure 6: Nrxn1SS4+ - Cbln2 signaling controls NMDAR-EPSCs but not AMPAR-EPSCs in the PFC, whereas Nrxn3SS4+-Cbln2 signaling does not regulate either AMPAR- or NMDAR-EPSCs in the PFC.

Figure 7: Constitutive Cbln2 deletion does not alter the overall synapse density in the PFC.

Figure 8: Nrxn3SS4+ - Cbln1 signaling controls AMPAR-EPSCs in the cerebellum, but in this brain region Nrxn1SS4+ - Cbln1 signaling has no effect.
Evidence tracking, Biological System:Intact tissue
Evidence tracking, Protein Targeting:Genetic transformation (eg; knockout, knockin, mutations)
Antibody (detection)
Evidence tracking, Experiment Assay:Confocal
Electrophysiology (generic)
Western blot
Annotator(s):Frank Koopmans (ORCID:0000-0002-4973-5732)
Guus Smit (ORCID:0000-0002-2286-1587)
Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
Additional literature:"structural and functional analyses of the prototypical molecular bridge linking post-synaptic iGluR δ2 (GluD2) and pre-synaptic β-neurexin-1 (β-NRX1) via Cbln1, a C1q-like synaptic organizer" @ PMID:27418511

Characterization of Cbln1, Cbln2, Cbln3, and Cbln4 expression in mouse brain showed differential expression patterns in brain regions and neuronal populations. @ PMID:28714144
SynGO annotation ID:4993
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology