| Annotated protein: | ELAV-like protein 4 (Hu-antigen D) (HuD) (Paraneoplastic encephalomyelitis antigen HuD). Gene symbol: ELAVL4. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: O09032 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ELAVL4 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of translation at postsynapse (GO:0140245) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Vanevski F, et al. "HuD interacts with Bdnf mRNA and is essential for activity-induced BDNF synthesis in dendrites" PLoS One. 2015 Feb 18;10(2):e0117264 PMID:25692578 |
| Figure(s): | Fig. 1 |
| Annotation description: | Fig. 5: HuD (ELAVL4) co-localises with Bdnf mRNA in hippocampal cultures transfected with recombinant GFP-mHuD at 14 DIV and stained using antisense probe against Bdnf. Furthermore knock-down of endogenous HuD in rat hippocampal cultures blocked the ability of PKC activation to increase dendritic translation of mRNAs with the long Bdnf 3' UTR. 28/5/2018 Pim - Accepted in SynGO because BDNF is a synaptically active compound: "Brain-derived neurotrophic factor (BDNF) is an important molecule involved in learning and memory in the adult brain, with demonstrated roles in regulating synaptic plasticity [1-3]. " |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Alexandros Kanellopoulos (ORCID:0000-0002-2094-7491) Vittoria Mariano (ORCID:0000-0002-9848-0262) Achsel Tilmann (ORCID:0000-0002-1190-4481) Claudia Bagni (ORCID:0000-0002-4419-210X) |
| Lab: | Department of Fundamental Neurosciences, University of Lausanne, CH-1006 Lausanne, Switzerland; Dept Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy |
| Additional literature: | Table 1S: The protein was detected in MS in mouse (M. musculus; 6-8-week-old 129 Strain) and zebrafish (D. rerio; adult male and females) whole brain synaptosomal preparations. @ PMID:28252024 Table T1: protein reproducibly detected in mouse synaptosome preparation. @ PMID:25211037 Fig. 1: Immunofluorescence on hippocampal primary neurons DIV14 showed localisation of HuD (ELAVL4) in post-synaptic compartments. The figure showed also co-localisation of HuD with eIF4E and PABP, HuD takes part in the translational complex at synapses. @ PMID:18769135 [human] --> overexpression of human gene electrophysiology experiment in mice over-expressing HuD, the results were: "Electrophysiological analyses of the mossy fiber to CA3 synapse of HuD-Tg mice revealed increases in paired-pulse facilitation (PPF) at short interpulse intervals and no change in long-term potentiation (LTP). " @ PMID:18493953 |
| SynGO annotation ID: | 499 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |