| Annotated protein: | E3 ubiquitin-protein ligase LNX (EC 2.3.2.27) (Ligand of Numb protein X 1) (Ligand of Numb-binding protein 1) (Numb-binding protein 1) (RING-type E3 ubiquitin transferase LNX). Gene symbol: LNX1. Taxonomy: Mus musculus (Mouse). Uniprot ID: O70263 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ LNX1 |
| Ontology domain: | Biological Process |
| SynGO term: | synapse maturation (GO:0060074) |
| Synapse type(s): | Mossy fiber synapse (DG->CA3) |
| Annotated paper: | Liu XD, et al. "Retrograde regulation of mossy fiber axon targeting and terminal maturation via postsynaptic Lnx1" J Cell Biol. 2018 Nov 5;217(11):4007-4024 PMID:30185604 |
| Figure(s): | Figure 3, 4, 5, 8 |
| Annotation description: | Figure 3: Defective presynaptic maturation and function in Lnx1-null mice. EM imaging of mossy-fiber synapses in wildtype and Lnx1 -/- mice showed deficits in PSD number per spine, docked synaptic vesicles per release site and synaptic vesicle density. Figure 4: Reverse IPs with knockout and mutated-domain controls showed Lnx1 and EphB1/2 can interact. Figure 5: Lnx1 stabilizes EphB receptors through specific binding sites to prevent their degradation in proteasome. Figure 8: The diminished axon terminals in Lnx1-/- mice (Figure 3, 8a) were restored in mice with both Lnx1-/- and EphB2F620D/F620D mutations. The latter is a constitutively active form of EphB tyrosine kinase. Taken together, authors showed that Lnx1 mediated EphB1 and EphB2 kinase activation promotes mossy fiber presynaptic terminal maturation (Figure 8H). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Electron Microscopy IP + WB/MSMS |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4986 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |