| Annotated protein: | Cartilage acidic protein 1 (68 kDa chondrocyte-expressed protein) (CEP-68) (ASPIC) (Protein CRTAC1-B). Gene symbol: CRTAC1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8R555 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CRTAC1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synapse assembly (GO:0051963) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Nishida R, et al. "LOTUS, an endogenous Nogo receptor antagonist, is involved in synapse and memory formation" Sci Rep. 2021 Mar 3;11(1):5085 PMID:33658590 |
| Figure(s): | Figure 2, 3 |
| Annotation description: | Figure 2: "To investigate the function of LOTUS in synapse formation, we measured the synaptic density via simultaneous immunostaining of the postsynaptic marker PSD-95 and the presynaptic marker Bassoon in cultured hippocampal neurons. The number of positive staining deposits was measured (Fig. 2a - d). The number of synapses was decreased in LOTUS-KO mice compared with WT mice (Fig. 2e). These data suggest that LOTUS contributes to synapse formation in cultured hippocampal neurons." Figure 3: "Mice in which dendritic spines can be visualized were created using Thy1-EGFP mice. Thus, the spine density of EGFP-positive dendrites was examined in the hippocampus of adult Thy1-EGFP WT and Thy1-EGFP LOTUS-KO mice. The apical dendritic spine density in hippocampal CA1 pyramidal neurons was significantly decreased in LOTUS-KO mice compared with WT mice. In particular, the number of mushroom-type and thin-type spines was significantly decreased in LOTUS-KO mice. However, no difference was observed in the number of stubby-type spines in these mice (Fig. 3a,b). Similarly, the same measurement was performed in basal dendrite, which yielded similar results to those obtained for apical dendrites (Fig. 3c,d). These results indicate that loss of LOTUS reduces the number of thin and mushroom-type spines in hippocampal CA1 pyramidal neurons, as well as the total spine density, suggesting that LOTUS may contribute to hippocampal synapse formation." note that LOTUS is an alias for gene CRTAC1, PSD-95 is an alias for gene DLG4. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4983 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |