| Annotated protein: | AP-2 complex subunit alpha. Gene symbol: APA-2. Taxonomy: Caenorhabditis elegans (Worm). Uniprot ID: Q22601 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ AP2A1 SynGO data @ AP2A2 |
| Ontology domain: | Biological Process |
| SynGO term: | synaptic vesicle endocytosis (GO:0048488) |
| Annotated paper: | Gu M, et al. "AP2 hemicomplexes contribute independently to synaptic vesicle endocytosis" Elife. 2013 Mar 5;2:e00190 PMID:23482940 |
| Figure(s): | Figure 7, 8 |
| Annotation description: | The AP2 adaptor complex mediates clathrin-dependent endocytosis of proteins localized to the cell membrane (PMID:21779028). The alpha-adaptin subunit, C. elegans gene apa-2 with mammalian orthologs AP2A1 and AP2A2 (both abundantly expressed in mammalian synapses, c.f. other SynGO annotations), is a core part of this protein complex (PMID:12086608). Figure 7, supplement 1: α-adaptin (apa-2) colocalizes with synaptobrevin in C.elegans at synapses in the dorsal nerve cord of GABA motor neurons. Figure 8: α-adaptin (apa-2) mutants show endocytic defects in acetylcholine neuromuscular junctions in the ventral nerve cord of C.elegans, as observed by reduced SV numbers, increased SV diameter and an increased number of large vesicles. Figure 8, supplements: Synaptic vesicles are reduced at GABA synapses in α-adaptin (apa-2) mutants and α-adaptin μ2-adaptin double mutants. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | structure and assembly of the AP2 core complex @ PMID:12086608 review paper describes the role of the AP2 complex in SV endocytosis @ PMID:21779028 |
| SynGO annotation ID: | 4976 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |