| Annotated protein: | Neuronal pentraxin-2 (NP2) (Neuronal pentraxin II) (NP-II). Gene symbol: NPTX2. Taxonomy: Mus musculus (Mouse). Uniprot ID: O70340 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NPTX2 |
| Ontology domain: | Cellular Component |
| SynGO term: | synapse (GO:0045202) |
| Synapse type(s): | cerebral cortex |
| Annotated paper: | Kovacs RA, et al. "Identification of Neuronal Pentraxins as Synaptic Binding Partners of C1q and the Involvement of NP1 in Synaptic Pruning in Adult Mice" Front Immunol. 2021 Feb 8;11:599771 PMID:33628204 |
| Figure(s): | Figure 2, 4 |
| Annotation description: | Figure 2: Mouse primary cortical neurons were immunostained for MAP2, synaptophysin, and neuronal pentraxin-2 (NPTX2 / NP2), which showed partial co-localization. Figure 4: High-resolution confocal microscopy images of triple immunostained cerebral cortical sections were segmented to identify individual C1q, NP2, and synaptophysin spots. NP2 (as well as NP1) showed extensive colocalization with both synaptophysin and C1q. Figure 5: in biochemical subfraction, NP2 was enriched in synaptic cytoplasm subfraction (as opposed to NP1 which was enriched in synaptic membrane fraction). |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Biochemical fractionation (generic) Western blot |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4969 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |