| Annotated protein: | Low-density lipoprotein receptor-related protein 4 (LRP-4) (LDLR dan). Gene symbol: LRP4. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8VI56 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ LRP4 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse assembly (GO:0150052) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Gomez AM, et al. "Synaptic plasticity and cognitive function are disrupted in the absence of Lrp4" Elife. 2014 Nov 19;3:e04287 PMID:25407677 |
| Figure(s): | Figure 5, 8 |
| Annotation description: | Figure 5: Paired-pulse facilitation was similar in control and Lrp4-/-; Lrp4m mice, indicating that Lrp4 is not essential for this form of evoked transmitter release and short-term plasticity (Figure 5B). However, when theta burst stimulation (TBS) was used to strongly activate synapses, authors found that TBS induced robust LTP from wild-type CA1 neurons (Figure 5G,L,N), whereas TBS failed to induce LTP in Lrp4-/- CA1 neurons (Figure 5H,M,N). Figure 8: A loss of Lrp4 decreases spine density in primary apical dendrites of CA1 neurons. "we generated mice that are mutant for Lrp4, carry the Lrp4m transgene that restores Lrp4 expression in muscle, and also carry the Thy1::YFP-H transgene, which sparsely labels neurons, to examine spine density along the apical-basal axis." ... "The spine density at several locations (basal, oblique apical, and tufts) was similar in Lrp4-/-; Lrp4m and wild-type mice (Figure 8B,C). However, the density of spines on primary apical dendrites on CA1 neurons was reduced (~20%) in Lrp4-/-; Lrp4m mice (Figure 8B,C). This decrease in spine density in CA1 neurons of Lrp4-/-; Lrp4m mice is consistent with the reduction in mEPSC frequency and impaired synaptic plasticity." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | agrin induces an Lrp4-dependent increase of excitatory synaptic puncta @ PMID:30385274 |
| SynGO annotation ID: | 4968 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |