| Annotated protein: | Dynamin-2 (EC 3.6.5.5). Gene symbol: DNM2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P39052 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DNM2 |
| Ontology domain: | Biological Process |
| SynGO term: | postsynaptic endocytosis (GO:0140239) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Scheefhals N, et al. "Shank Proteins Couple the Endocytic Zone to the Postsynaptic Density to Control Trafficking and Signaling of Metabotropic Glutamate Receptor 5" Cell Rep. 2019 Oct 8;29(2):258-269.e8 PMID:31597090 |
| Figure(s): | Figure 1 |
| Annotation description: | Figure 1a-b: authors developed an assay to image the surface expressed pool of mGluR5 (GRM5) in live cells and observe agonist-induced mGluR5 internalization in dendritic spines of hippocampal neurons. Figure 1j: the expression of a dominant-negative form of Dynamin2 (DNM2 / Dyn2), Dynamin2-K44A (Dyn2-K44A), reduced agonist-induced internalization of mGluR5 in dendritic spines. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4965 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |