| Annotated protein: | Guanine nucleotide-binding protein G(o) subunit alpha. Gene symbol: GNAO1. Taxonomy: Mus musculus (Mouse). Uniprot ID: P18872 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GNAO1 |
| Ontology domain: | Biological Process |
| SynGO term: | postsynaptic modulation of chemical synaptic transmission (GO:0099170) |
| Synapse type(s): | striatum |
| Annotated paper: | Muntean BS, et al. "Galphao is a major determinant of cAMP signaling in the pathophysiology of movement disorders" Cell Rep. 2021 Feb 2;34(5):108718 PMID:33535037 |
| Figure(s): | Figure 2 |
| Annotation description: | "Measurement of intrinsic membrane properties of iMSNs showed no significant alterations in firing properties between iMSN KO (Gnao1flox/flox:Drd2Cre) mice and WT controls (Drd2Cre) (Figures 2A-2C). In contrast, dMSN KO (Gnao1flox/flox:Drd1aCre) neurons had significantly increased excitability, as evidenced by a greater firing frequency (Figures 2D and 2E) and decrease in the rheobase current compared with control (Drd1aCre) (Figure 2F)." "Recordings of miniature excitatory postsynaptic currents (mEPSCs) revealed a significant increase in amplitude, but not frequency, in both iMSN KO and dMSN KO (Figures S2F-S2H) consistent with the postsynaptic nature of a change. ... deletion of Gαo significantly increased the AMPAR/NMDAR ratio in iMSN KO (Figure 2I) and dMSN KO (Figure 2J). We further ascertained the postsynaptic nature of observed changes by examining the dynamics of dopamine input onto MSNs using cyclic voltammetry and found no changes in evoked dopamine dynamics in either dMSN KO or iMSN KO slices (Figures S2K and S2L). Together, our electrophysiological data indicate that Gαo acts postsynaptically in MSNs to significantly impact synaptic properties of both MSN populations and selectively influence intrinsic excitability of dMSNs." dMSN; direct-pathway medium spiny neuron. iMSN; indirect-pathway medium spiny neurons. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4961 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |