| Annotated protein: | ADP-ribosylation factor 6 (EC 3.6.5.2). Gene symbol: ARF6. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P62332 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ARF6 |
| Ontology domain: | Biological Process |
| SynGO term: | synaptic vesicle endocytosis (GO:0048488) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Tagliatti E, et al. "Arf6 regulates the cycling and the readily releasable pool of synaptic vesicles at hippocampal synapse" Elife. 2016 Jan 5;5:e10116 PMID:26731518 |
| Figure(s): | Fig 1, Fig3, Fig 4 and Fig S3 |
| Annotation description: | Upon ARF6 knockdown, synaptic vesicles are depleted and endosome-like cisternae accumulate (Fig1). This can be reverted by expressing shRNA resistant ARF6 (Fig S3) or phenocopied by acute chemical ARF6GEF inhibition by SecinH3 (Fig 3), indication that this is really attributed to impaired AFR6 function. Blocking synaptic activity by TTX also reverts the synaptic vesicle and cisternae number, showing that synaptic activity is needed for the ARF6-dependent SV pool maintenance (Fig4). ARF6 could regulate SV endocytosis and recycling either directly at the plasma membrane and/or from the endosome-like cisternae. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Jan van Weering (ORCID:0000-0001-5259-4945) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 496 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |