| Annotated protein: | Guanine nucleotide-binding protein subunit beta-5 (Gbeta5) (Transducin beta chain 5). Gene symbol: GNB5. Taxonomy: Mus musculus (Mouse). Uniprot ID: P62881 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GNB5 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic membrane (GO:0045211) |
| Synapse type(s): | cerebellum Parallel fibre (cerebellum -> Purkinje-cell) |
| Annotated paper: | Aguado C, et al. "Cellular and Subcellular Localization of the RGS7/Gbeta5/R7BP Complex in the Cerebellar Cortex" Front Neuroanat. 2016 Nov 30;10:114 PMID:27965545 |
| Figure(s): | Fig.5 |
| Annotation description: | Fig.5: "Subcellular localization of RGS7, Gβ5 and R7BP in PCs" - "Immunoreactivity for RGS7 and Gβ5 showed virtually the same subcellular localization in PCs (Figures 5A,B,D,E). Immunoparticles for both proteins were primarily detected postsynaptically in dendritic shafts and dendritic spines of PCs (83.5% of RGS7; n = 4.417; and 82.8% of Gβ5; n = 2.253), with low labeling detected in presynaptic terminals and axons (16.5% of RGS7; n = 871; and 17.2% of Gβ5; n = 469; Figures 5A,B,D,E). Postsynaptically, labeling for RGS7 and Gβ5 was found primarily along the plasma membrane (28.9% of RGS7; n = 1.536; and 27.3% of Gβ5; n = 747) but also associated with the endoplasmic reticulum (ER) cisterna of dendritic shafts and spine apparatus (54.6% of RGS7; n = 2.899; and 55.5% of Gβ5; n = 1.516; Figures 5A,B,D,E). RGS7 and Gβ5 were observed primarily at extrasynaptic and perisynaptic positions in dendritic spines establishing synaptic contact with parallel fiber AT (Figures 5A,B,D,E)." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | rabbit anti-Gβ5 antibody characterized in this paper @ PMID:18842904 |
| SynGO annotation ID: | 4956 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |