| Annotated protein: | Synaptophysin (BM89 antigen) (Major synaptic vesicle protein p38). Gene symbol: SYP. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q62277 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SYP |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synaptic vesicle priming (GO:0010807) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Raja MK, et al. "Elevated synaptic vesicle release probability in synaptophysin/gyrin family quadruple knockouts" Elife. 2019 May 15;8:e40744 PMID:31090538 |
| Figure(s): | Fig 7c |
| Annotation description: | The authors developed an optical imaging assay in primary cell culture and first loaded the recycling synaptic vesicles with FM4-64 dye during 60 s of 20 Hz electrical stimulation (Gaffield and Betz, 2006; see Figure 7-figure supplement 1 for example images) and then monitored destaining with time-lapse fluorescence imaging during low frequency (0.2 Hz) stimulation in the absence of dye, followed by near complete destaining with a second 20 Hz train. The results indicate that destaining at 0.2Hz is significantly increased in synapses of different null mutants (SYP1,2 SYG1,3), most importantly between QKO and TKO + SYP1. Given other evidence in the same study (Fig 2-6), the data indicate that SYP1 is a negative regulator of exocytosis, and especially of fusogenicity, suggesting a role in the priming steps. Protein names; SYP1 = synaptophysin/SYP; SYP2 = synaptophysin-2 aka. synaptoporin/SYNPR; SYG1 = synaptogyrin-1/SYNGR1; SYG3 = synaptogyrin-3/SYNGR3. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Optical physiology |
| Annotator(s): | Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | original SYP null mutant mice @ PMID:8581936 |
| SynGO annotation ID: | 4954 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |