| Annotated protein: | Calcium-dependent secretion activator 2 (Calcium-dependent activator protein for secretion 2) (CAPS-2). Gene symbol: CADPS2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8BYR5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CADPS2 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | Parallel fibre (cerebellum -> Purkinje-cell) |
| Annotated paper: | Sadakata T, et al. "The secretory granule-associated protein CAPS2 regulates neurotrophin release and cell survival" J Neurosci. 2004 Jan 7;24(1):43-52 PMID:14715936 |
| Figure(s): | Fig. 5 |
| Annotation description: | Pre-embedding immunoelectron microscopy (EM) using a rabbit polyclonal anti-CAPS-2 antibody (anti-a1803/CAPS2 amino acids 18-89) generated in the study. No CAPS-2 KO were used to test antibody specificity for immuno-EM. 31/5/2017 Pim - The included immuno-EM pictures show much more detailed location on predantly non-specified presynaptic vesicular structures which are not SVs (for which SynGO has no term) but also on the presynaptic plasma membrane and in the dendritic spine. Since the majority of the protein is in the presynapse and the authors fail to address the nature of the presynaptic vesicle I agree to the term "presynapse". |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| Additional literature: | Characterization of the CAPS-2 antibody that was used in this study by immunohistochemistry. @ PMID:17344385 rat CAPS2[F1LWT1] Additional literature: PMID:18022372 --> Characterization of the anti-CAPS2 antibody using Western Blot of CAPS-2 KO homogenate. Fig.4C: Rat cortex subcellular fractions were analyzed by Western blotting using anti-CAPS-2 antibodies. CAPS-2 is a soluble protein that localizes to a large soluble synaptic pool in LS2 (cytosolic synaptosomal fraction) and a small membrane bound pool associated with the vesicle fraction LP2 (crude synaptic vesicle fraction). @ PMID:14530279 |
| SynGO annotation ID: | 495 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |