| Annotated protein: | Dystrophin. Gene symbol: DMD. Taxonomy: Mus musculus (Mouse). Uniprot ID: P11531-7 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DMD |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, GABAergic |
| Annotated paper: | Fujimoto T, et al. "Generation of dystrophin short product-specific tag-insertion mouse: distinct Dp71 glycoprotein complexes at inhibitory postsynapse and glia limitans" Cell Mol Life Sci. 2022 Jan 30;79(2):109 PMID:35098363 |
| Figure(s): | 3a-f, 5b-d, S6a |
| Annotation description: | Dp71 is a short product of Dystrophin (gene symbol DMD). Figure 3 and 5: HA-Dp71 colocalises with Gephyrin (GPHN), an inhibitory postsynapse marker, in the hippocampal dentate gyrus (DG) granular layer of the transgenic (TG) mouse brain section (Fig. 3) and cultured hippocampal neurons (Fig. 5). Authors write: "Taken together, HA-Dp71 was exclusively localized to inhibitory postsynapses in the cultured hippocampal neurons." WT vs HA-Dp71 control for HA-antibody specificity in Figure 2. Supplementary Figure 6a: "In agreement with all above results, HA-Dp71 puncta were adjacent to GABAergic presynaptic terminals labelled by GAD67" The gephyrin staining had cross reactivity with the nucleus in Figures 3 and 4, on which authors noted: "Arrows indicate strong cross-reactions against nuclear component revealed by anti-Gephyrin antibody which should be ignored". This brings the specificity of the gephyrin antibody into question. However, taken together with the presented co-localization with VGAT (Fig. 5c) and GAD67 (Fig. S6a), localization of Dp71 (DMD) to the postsynapse is likely. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Robin Clement Guus Smit Matthijs Verhage |
| ORCID: | 0000-0002-2286-1587 0000-0002-2514-0216 |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4938 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |