| Annotated protein: | MAM domain-containing glycosylphosphatidylinositol anchor protein 1 (Ig6M). Gene symbol: MDGA1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P85171 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ MDGA1 |
| Ontology domain: | Cellular Component |
| SynGO term: | anchored component of postsynaptic density membrane (GO:0099031) |
| Synapse type(s): | hippocampus, glutamatergic hippocampus, GABAergic |
| Annotated paper: | Toledo A, et al. "MDGAs are fast-diffusing molecules that delay excitatory synapse development by altering neuroligin behavior" Elife. 2022 May 9;11:e75233 PMID:35532105 |
| Figure(s): | Figure 1, 2 |
| Annotation description: | Figure 1: confocal imaging showed MDGA1 co-localizes with PSD95 (DLG4) and Gephyrin (GPHN) in cultured rat hippocampal neurons at DIV7/14/21. Related supplementary figures show validation of the antibody. "Overall, among synapses that contained MDGA1, the area overlap between PSD-95 and MDGA1, or between gephyrin and MDGA1 was around 15-30% (Figure 1H and K), pointing to a minor occupancy of both excitatory and inhibitory synapses by MDGA1." Figure 2: STORM imaging showed MDGA1 (the endogenous protein was replaced by recombinant tagged counterparts) co-localizes with Homer1c in cultured rat hippocampal neurons at DIV10 and DIV14. Related supplementary figures show validation of shRNA and rescue MDGA constructs in neuronal cultures. Authors note that "AP-MDGA1 and AP-MDGA2 displayed a fairly uniform distribution at DIV 10 and 14, filling the whole dendritic shaft without specific accumulation at synapses, similarly to the negative control GFP-GPI (Figure 2A and C)". MDGAs are GPI-anchored membrane molecules, hence "anchored component of ... membrane" term was selected. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Super resolution Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4926 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |