| Annotated protein: | Dipeptidyl aminopeptidase-like protein 6 (DPPX) (Dipeptidyl aminopeptidase-related protein) (Dipeptidyl peptidase 6) (Dipeptidyl peptidase IV-like protein) (Dipeptidyl peptidase VI) (DPP VI). Gene symbol: DPP6. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9Z218 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DPP6 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic membrane potential (GO:0060078) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Sun W, et al. "DPP6 establishes the A-type K(+) current gradient critical for the regulation of dendritic excitability in CA1 hippocampal neurons" Neuron. 2011 Sep 22;71(6):1102-15 PMID:21943606 |
| Figure(s): | Figure 2, 5A&C , 7E&G |
| Annotation description: | Figure 2A-B: A-Type K+ Current Density Gradient Is Abolished in DPP6-KO CA1 Dendrites. In panel A&B, authors show that DPP6-KO mice lack a dendritic A-type K+ current gradient using dendritic patch clamp recordings in (A) CA1 pyramidal neurons in acute hippocampal slices and (B) in cell-attached recordings from CA1 dendrites. Figure 2C-G: Kv2.4 recruitment to the distal dendrites is reduced in DPP6-KO mice compared to WT. This is demonstrated using (C-D) western blot of WT and DPP6-KO slices, (E-F) immunohistochemical labelling in sagittal hippocampal slices of WT and DPP6-KO mice, and (G) immunogold localization of Kv4.2 in the CA1 stratum radiatum of the hippocampus of WT and DPP6-KO mice Figure 5: current clamp experiments in dendritic whole-cell recordings of CA1 hippocampal neurons show that dendritic excitability is enhanced in DPP6-KO CA1 pyramidal neurons by demonstrating that in (A) back-propagating action potentials recorded in distal CA1 DPP6-KO dendrites were larger in amplitude than those recorded in dendrites from WT mice and in (C) that bAP amplitude exhibits a relatively small activity-dependent decrease in DPP6-KO. Figure 7: The pike-timing window for LTP Induction Is extended in DPP6-KO Mice, shown in: (E) an LTP magnitude - timing plot revealed that an expanded time window for LTP induction exists in DPP6-KO mice, (G) Compared with WT, DPP6-KO mice exhibited relatively larger single-burst integrals in the 36-40 and 41-45 ms time windows, indicating an extension of the spike-timing window of LTP induction. Given the demonstrated regulatory activity of DPP6 over the Kv2.4 channel, which influences the postsynaptic membrane potential, combined with the changes on dendritic excitability in the DPP6-KO model, the GO-term 'regulation of postsynaptic membrane potential' was selected. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Microscopy (generic) Whole-cell patch clamp Electrophysiology (generic) Western blot |
| Annotator(s): | Robin Clement Guus Smit Matthijs Verhage |
| ORCID: | 0000-0002-2286-1587 0000-0002-2514-0216 |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4923 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |