| Annotated protein: | Methyl-CpG-binding protein 2 (MeCp-2 protein) (MeCp2). Gene symbol: MECP2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q00566 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ MECP2 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synapse organization (GO:0050807) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Chapleau CA, et al. "Dendritic spine pathologies in hippocampal pyramidal neurons from Rett syndrome brain and after expression of Rett-associated MECP2 mutations" Neurobiol Dis. 2009 Aug;35(2):219-33 PMID:19442733 |
| Figure(s): | Fig. 4 |
| Annotation description: | Knockdown of endogenous MECP2 by MECP2 ShRNAi in rat hippocampal slice cultures (P7-P10) causes a reduction in the density of mature dendritic spines, dendritic nodes, total dendritic length and dendritic intersections at 4 days post transfection. MECP2 role for proper spine maturation is furthermore demonstrated in Fig. 2: Overexpression of RTT-associated MECP2 mutation causes persistent reduction in dendritic spine density in hippocampal pyramidal neurons. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Alexandros Kanellopoulos (ORCID:0000-0002-2094-7491) Vittoria Mariano (ORCID:0000-0002-9848-0262) Achsel Tilmann (ORCID:0000-0002-1190-4481) Claudia Bagni (ORCID:0000-0002-4419-210X) |
| Lab: | Department of Fundamental Neurosciences, University of Lausanne, CH-1006 Lausanne, Switzerland; Dept Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy |
| Additional literature: | Fig. 2: Quantitative analyses of dendritic spines in DiI-labeled CA1 pyramidal neurons of P7 mice carrying a mutation for MECP2 (Mecp2tm1.1Jae mutants) was lower compared to age-matched wildtype littermates (B6.Cg-Mecp2tm1.1Jae, Jaenisch strain; C57BL/6 background) @ PMID:22919518 Fig. 1 and 2: Golgi staining in layer 2/3 pyramidal neurons in Primary Somatosensory Cortex and Visual Cortex show that spine pruning is impaired in mice carrying duplication of MECP2 gene (FVB-Tg (MECP2)1Hzo/J;JAX Stock No: 008679) compared to the wild-type littermates (all on FVB background) at 3 months. @ PMID:28258509 Fig. 3: Quantitative analysis of synapse ultrastructure in hippocampal area CA1 showed an impairment in the vesicle pool docked at the active zones of both asymmetric spine synapses and symmetric shaft synapses of Mecp2 mutant mice compared to the wild-type littermate. (B6.Cg-Mecp2tm1.1Jae, "Jaenisch" strain; C57BL/6 background mice) @ PMID:21307327 Fig. 4: Activity-dependent vesicular release from the sucrose-loaded RRP of excitatory terminals in CA1 s. radiatum was significantly slower in hippocampal slices from Mecp2 mutant mice than in their wild-type littermates (P48-68; B6.Cg-Mecp2tm1.1Jae, "Jaenisch" strain; C57BL/6 background) ). @ PMID:21307327 Fig. 1; 3; 4: Whole-cell patch-clamp recordings from layer 5 (L5) pyramidal neurons in slices of the primary somatosensory cortex showed reduction in spontaneous firing, impairments in excitation and inhibition balance, reduced amplitude of excitatory quantal transmission in mice heterozygous for a Mecp2-mutant allele compared to the wild-type littermate (129, C57BL/6 and BALB/c) at both early symptomatic stages, and presymptomatic stages, as early as the second postnatal week. @ PMID:16116096 Table T1: protein reproducibly detected in mouse synaptosome preparation. @ PMID:25211037 |
| SynGO annotation ID: | 492 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |