| Annotated protein: | Calcium-dependent secretion activator 1 (Calcium-dependent activator protein for secretion 1) (CAPS-1) (rCAPS). Gene symbol: CADPS. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q62717 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CADPS |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | cerebellum, glutamatergic cerebral cortex |
| Annotated paper: | Speidel D, et al. "A family of Ca2+-dependent activator proteins for secretion: comparative analysis of structure, expression, localization, and function" J Biol Chem. 2003 Dec 26;278(52):52802-9 PMID:14530279 |
| Figure(s): | Fig. 6C, Fig. 4 C |
| Annotation description: | Fig.5 Pre-embedding immunoelectron microscopy using an anti-CAPS-1 antibody. The antibody (polyclonal antibody directed against rat CAPS1 was generated with GST-rCAPS1 (218-390) fusion protein as the antigen and affinity purified). The specificity of the antibody was tested by Western Blot using CAPS-1 KO brain homogenate in a later study. Specificity of the antibody for immunolabelling was not tested. Fig.4 Rat cortex subcellular fractions were analyzed by Western blotting using specific anti-CAPS-1 antibodies. CAPS-1 is a soluble protein that localizes to a large soluble synaptic pool in LS2 (cytosolic synaptosomal fraction) and a small membrane bound pool associated with the vesicle fraction LP2 (crude synaptic vesicle fraction). Antibody information from the M&M: Recombinant GST-rCAPS1 (218-390) fusion protein was generated by using the expression plasmid pGEX-rCAPS1 (218-390), which encodes rat CAPS1 residues 218-390 in frame with GST (18). A polyclonal antibody directed against rat CAPS1 was generated with GST-rCAPS1 (218-390) fusion protein as the antigen. The antiserum was affinity-purified on GST-rCAPS1 (218-390) and immobilized on an Immobilon-P polyvinylidene fluoride membrane (Millipore) (19). The antibody was mono-specific for CAPS1 and cross-reacted with the murine orthologue, as demonstrated by Western blots with wild-type and CAPS1-deficient brain tissue from a deletion mutant mouse. 12/5/2017 Pim: - Cytosol localization is not directly derived from the immuno-EM data, but inferred from refereced biochemical enrichement data (PMID:14530279, see 'Additional literature' section). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Western blot Biochemical fractionation (generic) |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| Additional literature: | Characterization of the CAPS-1 antibody for Western Blot using CAPS-1 KO mouse brain homogenate. @ PMID:15820695 SynGO ID #489: Rat cortex subcellular fractions were analyzed by Western blotting using specific anti-CAPS-1 antibodies. CAPS-1 is a soluble protein that localizes to a large soluble synaptic pool in LS2 (cytosolic synaptosomal fraction) and a small membrane bound pool associated with the vesicle fraction LP2 (crude synaptic vesicle fraction). @ PMID:14530279 |
| SynGO annotation ID: | 491 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |