| Annotated protein: | SLIT-ROBO Rho GTPase-activating protein 2 (srGAP2) (Formin-binding protein 2) (Formin-binding protein 27) (FBP-27). Gene symbol: SRGAP2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q91Z67 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SRGAP2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Charrier C, et al. "Inhibition of SRGAP2 function by its human-specific paralogs induces neoteny during spine maturation" Cell. 2012 May 11;149(4):923-35 PMID:22559944 |
| Figure(s): | figure 4a-b, figure S4 |
| Annotation description: | In Fig. 4A Srgap2 antibody staining shows a punctate pattern which overlaps with presynaptic marker Synapsin1 and excitatory postsynaptic marker Homer1. In Fig. 4B overexpressed Srgap2-mRFP shows colocalization with overexpressed Homer1c-GFP in dendritic spines. Figure S4 shows Immunoblots of homogenates and synaptic fractions from P15 mouse brains. SRGAP2 is enriched biochemical fractions of the postsynaptic density (PSD) similar to SAP102 (DLG3). |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Jessie Brunner Matthijs Verhage |
| ORCID: | 0000-0002-2514-0216 |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Fossatti et al. 2016 show in Figure 2 that Srgap2 colocalizes with excitatory postsynaptic marker Homer1 and inhibitory postsynaptic marker Gephryn. SRGAP2 interacts with Homer1 and Gephyrin in co-immunoprecipitation assays @ PMID:27373832 Yao et al. describe the generation of the C-terminal Srgap2 antibody in the methods section and show the alignment of the amino acid sequences of the low homologous domains of the respective srGAP, which were used to generate the antibodies (Fig. 1). @ PMID:17710530 |
| SynGO annotation ID: | 4900 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |