| Annotated protein: | TNF receptor-associated factor 6 (EC 2.3.2.27) (E3 ubiquitin-protein ligase TRAF6) (RING-type E3 ubiquitin transferase TRAF6). Gene symbol: TRAF6. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: B5DF45 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ TRAF6 |
| Ontology domain: | Biological Process |
| SynGO term: | synapse assembly (GO:0007416) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Vemula SK, et al. "The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development" Front Cell Dev Biol. 2020 Dec 9;8:579513 PMID:33363141 |
| Figure(s): | Figs 5, 6 |
| Annotation description: | TRAF6 mediates dendritic protrusion formation via neuroplastin (Fig. 5). Knockdown of TRAF6 via siRNA reduces number of formed synapses (panel D). Similarly, treatment with the TRAF6 inhibitor SMI 6860766 reduces the number of dendritic protrusions and Shank2 clusters per protrusion (H-N). Note, TRAF6 effects synapse formation only if applied before day in vitro 9 and not later (Fig. 4). TRAF6 blockage during neuronal development reduces synapse formation affecting neuronal activity (Fig. 6); treatment with SMI TRAF6 reduces the number of excitatory synapses and there is reduced network activity in SMI TRAF6-treated hippocampal neurons grown on multi-electrode arrays. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Antibody (detection) Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp Electrophysiology (generic) Confocal |
| Annotator(s): | Daniela Dieterich (ORCID:0000-0002-9880-1214) Rainer Pielot (ORCID:0000-0002-9681-3318) Karl-Heinz Smalla (ORCID:0000-0002-0269-0311) Eckart Gundelfinger (ORCID:0000-0001-9377-7414) |
| Lab: | Leibniz Institute for Neurobiology (LIN), Institute of Pharmacology and Toxicology, Otto von Guericke University, Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany |
| SynGO annotation ID: | 4899 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |