| Annotated protein: | TNF receptor-associated factor 6 (EC 2.3.2.27) (E3 ubiquitin-protein ligase TRAF6) (RING-type E3 ubiquitin transferase TRAF6). Gene symbol: TRAF6. Taxonomy: Mus musculus (Mouse). Uniprot ID: P70196 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ TRAF6 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of neurotransmitter receptor localization to postsynaptic specialization membrane (GO:0098696) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Ma Q, et al. "Proteasome-independent polyubiquitin linkage regulates synapse scaffolding, efficacy, and plasticity" Proc Natl Acad Sci U S A. 2017 Oct 10;114(41):E8760-E8769 PMID:28973854 |
| Figure(s): | Fig. 5D,E; Fig. 7G |
| Annotation description: | Traf6 is the E3 ubiquitin ligase that mediates constitutive K63 ubiquitination of PSD-95 (DLG4). Together with its deubiquitinase counterpart CYLD (SynGO annotation #4535) it controls PSD-95's clustering efficacy in spines and in turn controls cell surface availability of NMDA receptors in the postsynaptic membrane. Fig. 5D,E shows reduction of PSD-95 clustering upon Traf6 knock-down. Further: "Knocking down TRAF6 prevented the AP5-elicited increase of PSD-95 ubiquitination (Fig. 7G), supporting the notion that TRAF6 mediates constitutive K63 ubiquitination of PSD-95." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Confocal Western blot |
| Annotator(s): | Daniela Dieterich (ORCID:0000-0002-9880-1214) Rainer Pielot (ORCID:0000-0002-9681-3318) Karl-Heinz Smalla (ORCID:0000-0002-0269-0311) Eckart Gundelfinger (ORCID:0000-0001-9377-7414) |
| Lab: | Leibniz Institute for Neurobiology (LIN), Institute of Pharmacology and Toxicology, Otto von Guericke University, Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany |
| SynGO annotation ID: | 4898 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |