| Annotated protein: | Caspase-3 (CASP-3) (EC 3.4.22.56) (Apopain) (Cysteine protease CPP32) (CPP-32) (LICE) (Protein Yama) (SREBP cleavage activity 1) (SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12]. Gene symbol: CASP3. Taxonomy: Mus musculus (Mouse). Uniprot ID: P70677 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CASP3 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synaptic vesicle cycle (GO:0098693) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Gu Q, et al. "The BAD-BAX-Caspase-3 Cascade Modulates Synaptic Vesicle Pools via Autophagy" J Neurosci. 2021 Feb 10;41(6):1174-1190 PMID:33303681 |
| Figure(s): | Figure 1-3, 5, 6, 9 |
| Annotation description: | Figure 1: whole-cell voltage-clamp recordings of pyramidal neurons in hippocampal slices show diminished presynaptic release in BAD, BAX and caspase-3 (CASP3) KO mice, by demonstrating that (A) mEPSC frequency is reduced, (B) Paired-pulse ratio is increased and (C) EPSC amplitude is reduced in the different KO models compared to WT. Figure 2: whole cell recordings and electron microscopy were performed on hippocampal slices from BAD, BAX and caspase-3 KO mice. Analysis of sucrose evoked responses with electrophysiology, and the synaptic vesicle pool with EM shows evidence for a reduced readily releasable pool in the BAD, BAX and caspase-3 KO mice. Furthermore, authors show evidence for autophagy controlling the size of synaptic vesicle pools in presynaptic neurons (Figure 3, 5, 6) as well as evidence for activity-dependent regulation of autophagy by caspase-3, influencing presynaptic release following high-frequency stimulation (Figure 9). Given the role of caspase-3 in regulating the synaptic vesicle pool, here shown to be likely through the regulation of apoptosis through the BAD-BAX-caspase-3 cascade, the ontology term "regulation of synaptic vesicle cycle" was selected. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp Electrophysiology (generic) Electron Microscopy |
| Annotator(s): | Robin Clement Guus Smit Matthijs Verhage |
| ORCID: | 0000-0002-2286-1587 0000-0002-2514-0216 |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4891 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |