| Annotated protein: | Guanine nucleotide-binding protein subunit beta-5 (Gbeta5) (Transducin beta chain 5). Gene symbol: GNB5. Taxonomy: Mus musculus (Mouse). Uniprot ID: P62881 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GNB5 |
| Ontology domain: | Cellular Component |
| SynGO term: | synapse (GO:0045202) |
| Annotated paper: | Yim YY, et al. "Specificities of Gbetagamma subunits for the SNARE complex before and after stimulation of alpha(2a)-adrenergic receptors" Sci Signal. 2021 Dec 21;14(714):eabc4970 PMID:34932372 |
| Figure(s): | Figure 1, 2, 6 |
| Annotation description: | Figure 1a: experimental design; to determine the specificities of G-protein Gβγ subunits for the SNARE complex upon activation of α2a-Adrenergic Receptor (ADRA2A), authors used synaptosomes collected from the brains of wild-type, α2aAR KO, and FLAG-α2a-Adrenergic Receptor mice and co-IP of SNAP25 and Gβγ. Figure 2, 6: anti-SNAP25 pulldown yields Gβ5 (GNB5) and the relative amount thereof was affected by epinephrine stimulation to activate the α2a-Adrenergic Receptor. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Mass-spectrometry |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4880 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |