| Annotated protein: | Guanine nucleotide-binding protein G(i) subunit alpha-1 (Adenylate cyclase-inhibiting G alpha protein). Gene symbol: GNAI1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P10824 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GNAI1 |
| Ontology domain: | Biological Process |
| SynGO term: | neurotransmitter receptor localization to postsynaptic specialization membrane (GO:0099645) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Sans N, et al. "mPins modulates PSD-95 and SAP102 trafficking and influences NMDA receptor surface expression" Nat Cell Biol. 2005 Dec;7(12):1179-90 PMID:16299499 |
| Figure(s): | Figure 8 |
| Annotation description: | Figure 8: Gαi1 (GNAI1) is present in synaptic biochemical subfractions and co-localizes with a polyclonal antibody against PSD proteins (PSD-95, PSD-93, SAP97) in cultured hippocampal neurons. A pulldown of GDP-bound Gαi (pan antibody) yields postsynaptic proteins NR2B, NR1, SAP102 (DLG3), and over-expression of Gαi1 decreased surface expression (/puncta) of NR2B in dendrites of cultured hippocampal neurons. Taken together, Gαi(GNAI)-mPins(GPSM2) complex functions in trafficking of NR2B in hippocampal neurons. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) IP + WB/MSMS |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4870 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |