Annotated protein: | Guanine nucleotide-binding protein G(i) subunit alpha-1 (Adenylate cyclase-inhibiting G alpha protein). Gene symbol: GNAI1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P10824 |
antibody wiki: | |
SynGO gene info: | SynGO data @ GNAI1 |
Ontology domain: | Cellular Component |
SynGO term: | postsynapse (GO:0098794) |
Synapse type(s): | hippocampus, glutamatergic |
Annotated paper: | Sans N, et al. "mPins modulates PSD-95 and SAP102 trafficking and influences NMDA receptor surface expression" Nat Cell Biol. 2005 Dec;7(12):1179-90 PMID:16299499 |
Figure(s): | Figure 8 |
Annotation description: | Figure 8: Gαi1 (GNAI1) is present in synaptic biochemical subfractions and co-localizes with a polyclonal antibody against PSD proteins (PSD-95, PSD-93, SAP97) in cultured hippocampal neurons. A pulldown of GDP-bound Gαi (pan antibody) yields postsynaptic proteins NR2B, NR1, SAP102 (DLG3), and over-expression of Gαi1 decreased trafficking of NR2B. Taken together, this implies postsynaptic localization of Gαi1. |
Evidence tracking, Biological System: | Cultured neurons |
Evidence tracking, Protein Targeting: | Antibody (detection) |
Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) IP + WB/MSMS |
Non-tracable Author Statement (NAS): | This annotation required some inference over additional information/literature, and is not solely based on observations made in the annotated paper by its original authors. |
Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
SynGO annotation ID: | 4868 |
Dataset release (version): | 20231201 |
View annotation as GO-CAM model: |