Annotated protein:	Guanine nucleotide-binding protein G(o) subunit alpha. Gene symbol: GNAO1. Taxonomy: Mus musculus (Mouse). Uniprot ID: P18872
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SynGO gene info:	SynGO data @ GNAO1
Ontology domain:	Cellular Component
SynGO term:	postsynaptic membrane (GO:0045211)
Synapse type(s):	cerebellum, glutamatergic cerebellum, GABAergic Parallel fibre (cerebellum -> Purkinje-cell)
Annotated paper:	Roldan-Sastre A, et al. "Cellular Diversity and Differential Subcellular Localization of the G-Protein G(alphao) Subunit in the Mouse Cerebellum" Front Neuroanat. 2021 Jun 25;15:686279 PMID:34248508
Figure(s):	Figure 3, 5, 6
Annotation description:	Figure 3,5,6: pre- and post-embedding immunogold of cerebellar Purkinje cells showed GαO (GNAO1) was localized to postsynapses (mostly membrane, when PSD localized at its edges, some cytoplasmic) and to a lesser extend presynapses (mostly membrane, some cytoplasmic). Overall, the synaptic location of Gαo represented a small proportion compared to that located along the extrasynaptic plasma membrane and cytoplasmic sites. Authors note that this coincides with previous reporting on GABAb receptor localization, and showed that Gαo and GABAB1 were observed at PSDs of excitatory synapses and extrasynaptic sites between PC spines (s) and parallel fibers (pf), many times very close to each other.
Evidence tracking, Biological System:	Intact tissue
Evidence tracking, Protein Targeting:	Antibody (detection)
Evidence tracking, Experiment Assay:	Electron Microscopy
Annotator(s):	Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:	Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:	4865
Dataset release (version):	20231201
View annotation as GO-CAM model: