| Annotated protein: | Vesicle-associated membrane protein 4. Gene symbol: VAMP4. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: A0A096MJ99 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ VAMP4 |
| Ontology domain: | Biological Process |
| SynGO term: | neurotransmitter receptor transport, endosome to postsynaptic membrane (GO:0098887) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Bakr M, et al. "The vSNAREs VAMP2 and VAMP4 control recycling and intracellular sorting of post-synaptic receptors in neuronal dendrites" Cell Rep. 2021 Sep 7;36(10):109678 PMID:34496238 |
| Figure(s): | Figure 1, 2, 5 |
| Annotation description: | Figure 1: immunofluorescence and EM imaging show VAMP4 is a marker of recycling endosomes in soma and dendrites of hippocampal neurons. Figure 2: shRNA knockdown of VAMP4, but not VAMP2, impairs recycling endosome exocytosis and recycling to the plasma membrane. Figure 5: VAMP4 regulates the recycling of AMPA receptors and its availability for chemical-LTP Authors conclude: "These results show that AMPAR exocytosis depends on VAMP2, and that VAMP4 is necessary for the trafficking of AMPARs to a compartment that undergoes exocytosis after cLTP." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Confocal Electron Microscopy |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4863 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |