| Annotated protein: | Calcium/calmodulin-dependent protein kinase type II subunit gamma (CaM kinase II subunit gamma) (CaMK-II subunit gamma) (EC 2.7.11.17). Gene symbol: CAMK2G. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P11730 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CAMK2G |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic membrane neurotransmitter receptor levels (GO:0099072) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | He X, et al. "A Critical Role for gammaCaMKII in Decoding NMDA Signaling to Regulate AMPA Receptors in Putative Inhibitory Interneurons" Neurosci Bull. 2022 Aug;38(8):916-926 PMID:35290589 |
| Figure(s): | Figure 5, 6 |
| Annotation description: | "We found that cLTP increased the surface GluA1 level in putative inhibitory interneurons; these changes were prevented by knocking down γCaMKII with shRNA (Fig. 5). To investigate this process further, we monitored the phosphorylation of the AMPAR subunit GluA1 at Ser831 (pGluA1), the key step downstream of CaMKII activation during LTPE→E induction in excitatory neurons [2, 3]. We found that the γCaMKII shRNA, but not its scrambled control, inhibited cLTP-induced changes in pGluA1 (Fig. 6). Finally, the necessity of γCaMKII in the cLTP of inhibitory interneurons was probed by an shRNA-resistant construct (γCaMKIIR). With endogenous γCaMKII knocked down, 'wild-type' γCaMKIIR fully rescued pGluA1 and the surface GluA1 level (Figs. 5, 6). In contrast, γCaMKIIR K43R (the kinase-dead form [20]) was unable to rescue them (Figs. 5, 6), indicating that the kinase activity of γCaMKII is required to regulate AMPARs during cLTP of inhibitory interneurons." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4858 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |