| Annotated protein: | Sorting nexin-6 [Cleaved into: Sorting nexin-6, N-terminally processed]. Gene symbol: SNX6. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q6P8X1 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SNX6 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic early endosome (GO:0098842) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Niu Y, et al. "Ablation of SNX6 leads to defects in synaptic function of CA1 pyramidal neurons and spatial memory" Elife. 2017 Jan 30;6:e20991 PMID:28134614 |
| Figure(s): | Figure 4 |
| Annotation description: | Figure 4a,b: Immunofluorescence imaging showed SNX6 co-localizes with multiple endosomal marker proteins in dendrites of cultured hippocampal neurons. Figure 4e-i: SNX6 interacts with Homer1b/c. Figure 4j-n: EEA1, SNX6 and Homer1b/c triple-stainings suggest all three co-localize in early endosomes at dendrites of cultured hippocampal neurons. Punta in panel j suggest these endosomes are occasionally localized at/near dendritic spines. Figure 8: Homer1b/c translocates from shaft to spines by protein diffusion, which does not require SNX6. There is only very sporadic imaging data that places the SNX6 containing early endosomes (marked by EEA1) directly at the postsynapse. However, in order to deliver Homer1b/c to the postsynapse (by means of protein diffusion) these have to be very close at least i.e. to postsynaptic spine base/neck, which warrants annotation against postsynaptic endosomes as defined by SynGO. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | "one of SNX1/2's dimerization partners, SNX6, interacts with the p150(Glued) subunit of the dynein/dynactin motor complex. We present evidence that SNX6 is a component of the retromer, and that recruitment of the motor complex to the membrane-associated retromer requires the SNX6-p150(Glued) interaction." @ PMID:19935774 role of SNX6 in sorting endosomal cargo @ PMID:31576058 |
| SynGO annotation ID: | 4854 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |