| Annotated protein: | Ras-related protein Rab-7a (EC 3.6.5.2) (Ras-related protein BRL-RAS) (Ras-related protein p23). Gene symbol: RAB7A. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P09527 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RAB7A |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic endosome (GO:0098830) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Ackermann F, et al. "Critical role for Piccolo in synaptic vesicle retrieval" Elife. 2019 May 10;8:e46629 PMID:31074746 |
| Figure(s): | Figure 4 |
| Annotation description: | Rab7 is a marker for late endosomes (Zerial and McBride, 2001. PMID:11252952) Figure 4: co-localization of Synaptophysin (SYP) and GFP-Rab7 in cultured hippocampal neurons. Piccolo (PCLO) mutants disrupted endosomal Rab5 and Rab7 pathways for SV recycling. Authors concluded that "the maturation of membranes within the endosome compartment from Rab5 positive early endosomes towards late Rab7 positive endosomes is affected by the loss of Piccolo (Figure 4A)." From this we infer presynaptic localization of Rab7-positive endosomes, which cannot be directly observed from the imaging data (resolution too low). |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Non-tracable Author Statement (NAS): | This annotation required some inference over additional information/literature, and is not solely based on observations made in the annotated paper by its original authors. |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | direct observation of two cargo-specific membrane protein degradation mechanisms at axon terminals based on a live-imaging approach in intact Drosophila brains. Among several Rabs, RAB7 endosomal recycling at the presynapse is shown. @ PMID:29551411 Rab7 is a marker for late endosomes @ PMID:11252952 |
| SynGO annotation ID: | 4842 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |