| Annotated protein: | Profilin-2 (Profilin II). Gene symbol: PFN2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9JJV2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PFN2 |
| Ontology domain: | Biological Process |
| SynGO term: | presynaptic actin cytoskeleton organization (GO:0099140) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Waites CL, et al. "Piccolo regulates the dynamic assembly of presynaptic F-actin" J Neurosci. 2011 Oct 5;31(40):14250-63 PMID:21976510 |
| Figure(s): | Figure 8, 9 |
| Annotation description: | Figure 8: shRNA against Profilin-2 (PFN2) efficiently eliminated Profilin-2 from neurons without significantly affecting dendritic arborization or spine formation, the localization of EGFP-Syn at presynaptic boutons, or FM4-64 uptake. Figure 9: Using the high-K+/FM4-64 loading assay described above (pharmacological stimulus of actin clustering), we subsequently confirmed that presynaptic F-actin polymerization was impaired in neurons lacking Profilin-2. As anticipated, these neurons exhibited more diffuse EGFP-actin expression in unstimulated axons, as well as dramatically reduced stimulation-induced clustering of EGFP-actin. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4840 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |