| Annotated protein: | Gamma-aminobutyric acid receptor-associated protein (GABA(A) receptor-associated protein). Gene symbol: GABARAP. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9DCD6 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GABARAP |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of neurotransmitter receptor localization to postsynaptic specialization membrane (GO:0098696) |
| Synapse type(s): | cerebral cortex, GABAergic |
| Annotated paper: | Ye J, et al. "Structural basis of GABARAP-mediated GABA(A) receptor trafficking and functions on GABAergic synaptic transmission" Nat Commun. 2021 Jan 12;12(1):297 PMID:33436612 |
| Figure(s): | Figure 1, 2, 5, 7 |
| Annotation description: | Figure 1,2: GABARAP and γ2-GABAAR (GABRG2) were shown to interact in ITC and pulldown assays. Figure 5: GABARAP functions to stabilize GABAARs via promoting its trafficking pathway. Authors evaluated the effects of GABARAP on receptor trafficking in HEK-293 cells by treatment with Brefeldin A (BFA), which induces Golgi disassembly and is widely used to block the trafficking of membrane proteins. Reduced GABA current densities suggested a decrease in GABAAR membrane localization (Fig. 5b). Further, BFA treatment blocked the GABARAP-mediated increase in GABA currents as induced by 1 mM GABA (Fig. 5b), a finding demonstrating that GABARAP promotes receptor membrane localization via a trafficking pathway susceptibly regulated by BFA. Figure 7: In vivo disruption of GABARAP-GABAAR complex formation in M1 pyramidal neurons deleteriously impacts GABAergic synaptic activity. |
| Evidence tracking, Biological System: | Intact tissue Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Over-expression Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Protein-protein interaction (generic) Whole-cell patch clamp |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | mutations in GABARAP binding domain of GABAAR gamma2 distrupt GABAAR trafficking and GABARAP localization with NSF @ PMID:15601949 |
| SynGO annotation ID: | 4834 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |