| Annotated protein: | Gamma-aminobutyric acid receptor-associated protein (GABA(A) receptor-associated protein). Gene symbol: GABARAP. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P60517 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GABARAP |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic specialization, intracellular component (GO:0099091) |
| Synapse type(s): | hippocampus, GABAergic |
| Annotated paper: | Kittler JT, et al. "The subcellular distribution of GABARAP and its ability to interact with NSF suggest a role for this protein in the intracellular transport of GABA(A) receptors" Mol Cell Neurosci. 2001 Jul;18(1):13-25 PMID:11461150 |
| Figure(s): | Figures 1-8 |
| Annotation description: | Figure 1: immunofluorescence and confocal microscopy of cultured hippocampal neurons showed GABARAP was localized to both somatodendritic and axonal compartments of neurons. Figure 2: similar imaging for GABARAP co-stained with synaptophysin (SYP) / GABAa receptor gamma2 subunit (GABRG2) / gephryn (GPHN), showed that overlap between GABARAP and SYP ("synaptic GABARAP"). Figure 4: immunogold labeling of GABARAP in dendrites and axons showed localization to postsynaptic cytoplasm, dendrites, axons (but not presynaptic terminals). Additional immunoperoxidase labeling of GPHN showed presence in the same postsynapse, but no co-localization (GPHN at the membrane facing the synaptic cleft, GABARAP in at various distances of GPHN seemingly at intracellular membrane structures). Figure 5: immunogold labeling of GABARAP at neuronal somata showed localization to the Golgi apparatus and endoplasmic reticulum. Figure 7c: immunofluorescence and confocal microscopy imaging for GABARAP co-stained with NSF showed strong overlap throughout the dendrite. Furthermore, GST IP+WB showed that GABARAP and NSF interact (Figure 6). Figure 8. immunogold labeling of GABARAP and immunoperoxidase labeling of NSF showed co-localization at the edge of the Golgi apparatus and at postsynaptic cisternae. "Such postsynaptic cisternae have been shown to contain many components of the protein synthetic machinery (Gardiol et al., 1999) or alternatively may be part of postsynaptic tubulovesicular endosomes" |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4832 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |