| Annotated protein: | Band 4.1-like protein 1 (Erythrocyte membrane protein band 4.1-like 1) (Neuronal protein 4.1) (4.1N). Gene symbol: EPB41L1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9Z2H5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ EPB41L1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of exocytic insertion of neurotransmitter receptor to postsynaptic membrane (GO:0099145) |
| Annotated paper: | Lin DT, et al. "Regulation of AMPA receptor extrasynaptic insertion by 4.1N, phosphorylation and palmitoylation" Nat Neurosci. 2009 Jul;12(7):879-87 PMID:19503082 |
| Figure(s): | Figure 3, 4, 6, 7, 8 |
| Annotation description: | Besides associating with actin cytoskeleton, protein 4.1N (EPB41L1) binds specifically to the membrane proximal region (MPR) of GluR1 but not GluR2 (PMID:12574408, PMID:11050113). Figure 3,4: Protein 4.1N (EPB41L1) is required for activity dependent GluR1 (GluA1/GRIA1) insertion. Figure 6: de-palmitoylation of the C811 residue facilitates phosphorylation within GluR1 MPR by PKC, which in turn enhances the interaction between 4.1N and GluR1. Figure 7,8: siRNA knockdown of protein 4.1N reduced expression levels of endogenous GluR1 containing AMPARs. Furthermore, 4.1N is required for LTP expression in acute hippocampal slices of adult mice. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp Western blot IP + WB/MSMS |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | "Our findings demonstrate a novel molecular interaction for the GluR-D subunit and suggest that the association with the 4.1 family protein(s) plays an essential role in the transport to and stabilization of GluR-D-containing AMPA receptors at the cell surface." @ PMID:12574408 "These results suggest that protein 4.1N may link AMPA receptors to the actin cytoskeleton." @ PMID:11050113 "C-terminal palmitoylation decreases interaction of the AMPA receptor with the 4.1N protein and regulates AMPA- and NMDA-induced AMPA receptor internalization" @ PMID:16129400 |
| SynGO annotation ID: | 4828 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |