| Annotated protein: | Solute carrier family 12 member 5 (Electroneutral potassium-chloride cotransporter 2) (Furosemide-sensitive K-Cl cotransporter) (K-Cl cotransporter 2) (rKCC2) (Neuronal K-Cl cotransporter). Gene symbol: SLC12A5. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q63633 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SLC12A5 |
| Ontology domain: | Biological Process |
| SynGO term: | postsynaptic neurotransmitter receptor diffusion trapping (GO:0098970) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Gauvain G, et al. "The neuronal K-Cl cotransporter KCC2 influences postsynaptic AMPA receptor content and lateral diffusion in dendritic spines" Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):15474-9 PMID:21878564 |
| Figure(s): | Figure 5 |
| Annotation description: | Figure 5: Monitoring AMPA receptor lateral diffusion with quantum dot-based single-particle tracking showed that KCC2 (SLC12A5) suppression increases the lateral diffusion of GluR1 (GRIA1) in dendritic spines. KCC2 extinction did not affect the exploratory behavior or confinement of slow receptors in spines (Fig. 5 A and C). KCC2 apparently constrains, directly or indirectly, lateral movements of rapid GluR1-containing AMPA receptors in dendritic spines. This effect seems to be independent of changes in spine head diameter, because control experiments showed that lateral diffusion of GluR1 was unrelated to spine size (Fig. S2). In contrast to its effect on GluR1 diffusion in spines, KCC2 suppression did not significantly alter diffusion on dendritic shafts. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Reports a mechanism of KCC2 regulation by GABAA receptor (GABAAR)-mediated transmission in mature hippocampal neurons. Enhancing GABAAR-mediated inhibition confines KCC2 to the plasma membrane, while antagonizing inhibition reduces KCC2 surface expression by increasing the lateral diffusion and endocytosis of the transporter. @ PMID:29176664 KCC2 escapes inhibitory synapses faster than excitatory synapses, reflecting stronger molecular constraints at the latter. Interfering with KCC2-actin interactions or inhibiting F-actin polymerization releases diffusion constraints on KCC2 at excitatory but not inhibitory synapses. Thus, F-actin constrains KCC2 diffusion at excitatory synapses, whereas KCC2 is confined at inhibitory synapses by a distinct mechanism. @ PMID:24068817 |
| SynGO annotation ID: | 4825 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |