| Annotated protein: | Solute carrier family 12 member 5 (Electroneutral potassium-chloride cotransporter 2) (Furosemide-sensitive K-Cl cotransporter) (K-Cl cotransporter 2) (rKCC2) (Neuronal K-Cl cotransporter). Gene symbol: SLC12A5. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q63633 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SLC12A5 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse assembly (GO:0150052) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Li H, et al. "KCC2 interacts with the dendritic cytoskeleton to promote spine development" Neuron. 2007 Dec 20;56(6):1019-33 PMID:18093524 |
| Figure(s): | Figure 1, 2, 5 |
| Annotation description: | Figure 1: Dendritic Protrusions Are Significantly Longer in KCC2 (SLC12A5) -/- than in WT Neurons Figure 2: Reduced Number of Functional Synapses in Dendrites of KCC2 (SLC12A5) -/- Neurons Figure 5: Transfecting cultured WT neurons with a construct containing the KCC2-CTD only (aa 637-1116) induced long filopodia-like dendritic protrusions. "These results indicate that the KCC2-CTD mutant has a dominant-negative effect that leads to the disruption of intracellular interactions that are necessary for normal dendritic spine morphogenesis." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Reports a mechanism of KCC2 regulation by GABAA receptor (GABAAR)-mediated transmission in mature hippocampal neurons. Enhancing GABAAR-mediated inhibition confines KCC2 to the plasma membrane, while antagonizing inhibition reduces KCC2 surface expression by increasing the lateral diffusion and endocytosis of the transporter. @ PMID:29176664 KCC2 escapes inhibitory synapses faster than excitatory synapses, reflecting stronger molecular constraints at the latter. Interfering with KCC2-actin interactions or inhibiting F-actin polymerization releases diffusion constraints on KCC2 at excitatory but not inhibitory synapses. Thus, F-actin constrains KCC2 diffusion at excitatory synapses, whereas KCC2 is confined at inhibitory synapses by a distinct mechanism. @ PMID:24068817 |
| SynGO annotation ID: | 4824 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |