| Annotated protein: | Solute carrier family 12 member 5 (Electroneutral potassium-chloride cotransporter 2) (K-Cl cotransporter 2) (mKCC2) (Neuronal K-Cl cotransporter). Gene symbol: SLC12A5. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q91V14 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SLC12A5 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic specialization membrane (GO:0099060) |
| Annotated paper: | Hubner CA, et al. "Disruption of KCC2 reveals an essential role of K-Cl cotransport already in early synaptic inhibition" Neuron. 2001 May;30(2):515-24 PMID:11395011 |
| Figure(s): | Figure 3 |
| Annotation description: | Figure 3i: Immunofluorescence imaging showed co-localization of KCC2 (SLC12A5) and gephyrin (marker for inhibitory postsynapses). Figure 3j,k: "Electron microscopy on WT adult spinal cord confirms accumulation of KCC2 along the plasma membrane of dendrites but not in axons. The ultrastructure of a spinal cord dendrite revealed that immunogold-labeled KCC2 (asterisks) is located in proximity to synapses." authors conclude "Thus, KCC2 is predominantly, but not exclusively, localized at gephyrin-positive inhibitory synapses ... KCC2 was expressed at synapses (Figure 3K). It was enriched at the periphery of the postsynaptic specialization, whereas gephyrin is known to form a postsynaptic matrix that coextends with the active zone." Based on the known transmembrane topology of this protein, "integral component of ... membrane" term was selected. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Figure 1, 3; KCC2 is enriched at synapses. Authors find similar co-localization with glutamatergic postsynapses (homer1c-GFP) and GABAergic postsynapses (gephyrin-mRFP) @ PMID:24068817 |
| SynGO annotation ID: | 4822 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |