| Annotated protein: | Inhibitory synaptic factor 1 (InSyn1). Gene symbol: INSYN1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8CD60 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ INSYN1 |
| Ontology domain: | Biological Process |
| SynGO term: | postsynaptic specialization organization (GO:0099084) |
| Annotated paper: | Uezu A, et al. "Essential role for InSyn1 in dystroglycan complex integrity and cognitive behaviors in mice" Elife. 2019 Dec 12;8:e50712 PMID:31829939 |
| Figure(s): | Figure 1, 2, 5 |
| Annotation description: | Figure 1: InSyn1 localization to the iPSD is DGC dependent. InSyn1-HA localization after αDG (alpha-dystroglycan) or gephyrin CRISPR depletion. Neurons were depleted of αDG or gephyrin, followed by AAV:InSyn1-HA transduction 3 days before fixation. Figure 2: The N-terminus of InSyn1 is essential for its localization and interaction with DGC (dystroglycan complex) components. Figure 5: Altered distribution of GABAAR clustering in InSyn1 KO neurons. Authors conclude that "InSyn1 has a subunit-specific effect on GABAARs distribution in hippocampal neurons" |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Over-expression |
| Evidence tracking, Experiment Assay: | Confocal IP + WB/MSMS |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4814 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |