| Annotated protein: | Guanine nucleotide exchange factor C9orf72 homolog. Gene symbol: C9ORF72. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q6DFW0 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ C9ORF72 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | forebrain, glutamatergic Mossy fiber synapse (DG->CA3) |
| Annotated paper: | Xiao S, et al. "Synaptic localization of C9orf72 regulates post-synaptic glutamate receptor 1 levels" Acta Neuropathol Commun. 2019 Oct 24;7(1):161 PMID:31651360 |
| Figure(s): | Figure 1, 2 |
| Annotation description: | Figure 1: biochemical fractions of mouse forebrain show C9orf72 to be localized to both the pre- and post-synaptic compartment. Figure 2: IP+WB of PSD95 (DLG4) yields C9orf72 and Rab39b (Fig. 2a). Immunofluorescent imaging from C9-WT mossy fiber synapses demonstrated partial overlap of C9orf72 with pre-synaptic (Synpr), post-synaptic (PSD-95), and dendritic (MAP 2) proteins (Fig. 2c). C9orf72 was partially co-localized with Rab39b in the mossy fiber synapse region of C9-WT mice (Fig. 2c), indicating that C9orf72 shares synaptic localization with previously identified interactors. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4809 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |